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AN/DN NBLAST left/right comparison proofreading flags Step 1: Open sheet. Step 2: Find an index with ‘0’ in BOTH the ‘comparison_root_id_latest’ and ‘comparison_root_id’ columns. (Starts at index 484) Step 3: Put your name(first) and date(after competition) in column ‘M’ and ‘N’ respectively. Step 4: Open the hyperlink from ‘K’ column. Step 4a: Deselect and reselect the segment you are proofreading to ensure you have an updated segment. Step 5: Begin proofreading. Try to only spend 20-45 mins per cell - Unless it’s quite large and needs more time. Step 6: Once you have proofread all you can, use columns ‘P’ and ‘Q’ to select the drop down choices that match. P Column Choices ‘Proofreading needed’: Cell needed proofreading. (likely the case) ‘Not a good match (Cells are from different types)’: Please do NOT use this tag since there will only be one cell to proofread. ‘Can't find any proofreading error’: If there are no errors to fix. Q Column Choices ‘Done’: When you’re finished proofreading. ‘No need for proofreading’: Cell didn’t need proofreading. ‘Need 2nd opinion_proofreading’: Cell has a tough to resolve issue. (Note there is a similar tag “Need 2nd opinion_not sure a good match”. Please do NOT mix up with this one) ‘Can't fix (left)’ or ‘Can’t fix (right)’: If you’re cell has a major issue that prevents proofreading label it with the appropriate side ‘Can’t Fix’ Label. Step 7: Share your final link in the ‘O’ column. Step 8: Mark "backbone proofread" in banc-bot.

Which side is my Neuron on? To find out which side your neuron is on for the “Can't fix (right) and Can't fix (left)’ selection use this criteria. -Use the 3D mesh as a guide. -Place the mesh like this with the bottom half pointing to the right. -If your cell is on the right side of the mesh it’s ‘Right’ and if on the left side it’s ‘Left’.

Fixing Review Notes -Column ‘S’ might have notes from the reviewer with missed branches or mergers etc. -Please take a look at these notes and make the necessary adjustments to your cell(s), tags, and to the final links. -Once you have edited your cell(s) please make sure to use the ‘Fixed’ tag in column ‘T’. -If there is an issue with the review please use the ‘Can’t Fix’ tag. Example: